The study of the relation between triptolide inhibits peripheral blood mononuclear cell to secret TNF-? and tumour necrosis factor-? gene polymorphism
- VernacularTitle:TNF-?基因多态性对雷公藤甲素抑制PBMC分泌TNF-?的影响
- Author:
Hongbo CHEN
;
Shenghao TU
;
Dongyun SHENG
;
Yonghong HU
- Publication Type:Journal Article
- Keywords:
Triptolide;
Tumor necrosis factor TNF-?;
PBMC;
Gene polymorphism
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the relation between triptolide inhibit peripheral blood mononuclear cell to secret TNF-? and tumour necrosis factor-? gene polymorphism.Methods:Genomic DNA from 41 healthy people was typed for TNF-? -308 polymorphism by allele-specific polymorphism chain reaction(AS-PCR); the TNF-? concentration in the supernatant was measured by ELISA.Results:The TNF-? production of TNF-? -308 non-G/G genotype in LPS-inhibited peripheral blood mononuclear cell(PBMC) culture was more than that of G/G genotype; Compared with TNF-? -308 non-G/G genotype peripheral blood mononuclear cell(PBMC), triptolide can lower the production of TNF-? in G/G genotype peripheral blood mononuclear cell(PBMC).Conclusion:Tumour necrosis factor ?(TNF-?) gene polymorphism might influence the TNF-? secretion of peripheral blood mononuclear cell(PBMC) in healthy humans. We speculate that it may be relative to the different curative effect of Tripterygium Wilfordii Hook.F.(TWHF) to RA patients.
