The construction of phIFN-?-2B shuttle plasmid and its expression in BCG
- VernacularTitle:phIFN-?-2B穿梭质粒在卡介苗中的表达
- Author:
Chunyu LIU
;
Ruifa HAN
;
Tengxiang MA
;
Jiwu CHANG
;
Yuzhi HAN
;
Zhifang SUI
- Publication Type:Journal Article
- Keywords:
BCG;
Interferon;
Recombinant
- From:
Chinese Journal of Urology
2006;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a new secreting recombinant hIFN-?-2B-BCG to provide a new tool for the treatment of bladder tumor. Methods BCG was genetically engineered to secrete recombinant human interferon-alpha 2B by transforming of shuttle plasmid phIFN-?-2B. Expression of hIFN-? was readily detectable by ELISA. Results The phIFN-?-2B was transformed in BCG correctly,and the value of hIFN-?-2B in supernatant of recombinant BCG culture was calculated to approximately 997.2 pg/ml. Conclusions This study demonstrates that the recombinant phIFN-?-2B can be expressed in BCG secretively. As the construction of the plasmid and its transformation and expression in BCG were accomplished successfully, a foundation of reformed BCG and new vaccine will be established.
