DC loaded with CPP-Id increase the efficiency of CTL proliferation and enhance its cytotoxicity in vitro
- VernacularTitle:CPP-Id体外冲击DC对CTL增殖及杀伤活性的诱导作用
- Author:
Jianhua CHANG
;
Yanxia SHI
;
Jin LI
;
Xiaoshi ZHANG
;
Zhongzhen GUAN
- Publication Type:Journal Article
- Keywords:
mix lymphocyte reaction;
proliferation rate;
cytotoxocity
- From:
China Oncology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Dendritic cells(DCs) possess specialized feature such as pathogen recognition,antigen capturing and processing machinery,and stimulating naive T lymphocyte to have antitumor ability that allow them to act like professional APCs.This paper is aimed to confirm the impacts on the proliferation and secretion of INF-? of tumor specific CTL and its cytotoxocity induced by DC loaded with different antigen.Methods:After the stimulation of DC loaded with different antigen,the proliferative rate of allolymphocytes was measured by MTT and the cytotoxocity of CTL was evaluated with LDH method.The INF-? secreted by activated T lymphocytes was detected by ELISPOT.Results:The DC loaded with CPP-Id(320%?15%) had significantly induce T lymphocyte proliferating when comparing with the induction by DC loaded with Id(57%?10%)(P
