The study on the apoptosis of introduction p53 gene alone and incombination with TNF-? into Hep3B
- VernacularTitle:野生型p53基因联合TNF-?对Hep3B凋亡作用的研究
- Author:
Shurui BU
;
Lijuan HUO
;
Jinchun LIU
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Introduction p53 gene;
TNF-?
- From:
Cancer Research and Clinic
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the suppression effects of tumor suppressor gene p53 alone and apoptosis incombination with TNF-? in a hepatocellular carcinoma cell line Hep3B. Methods Hep3B cells were transfected with a wild-type p53 cDNA(wt-p53)and plain vector(pNeo) respectively. Then the cell were cultured for 12 hours, one of the transfected p53 groups was added TNF-?(20 ?g/ml). The expression of p53 was detected by immunological fluorescence assay. Determination of apoptosis was used by DNA fragmentation, TUNEL assay. Results Both a small dose TNF-? and wt-p53 can induce apoptosis more efficiently comparing with non-transfected cultures(P
