Expression and signififcance of IGF-Ⅰ TGF-?1 in human mesenchymal stem cells after exposured to extracoporeal shock wave
- VernacularTitle:冲击波干预成人骨髓间充质干细胞后IGF-Ⅰ和TGF-?1的表达及意义
- Author:
Wuzhou WANG
;
Gengyan XING
;
Qibin YE
- Publication Type:Journal Article
- Keywords:
Extracorporeal Shock Wave(ESW);
Human mesencbymal stem cells(hMSCs), Insulin-like growth Factor-Ⅰ(IGF-Ⅰ);
Transforming Growth Factor-?1(TGF-?1)
- From:
Orthopedic Journal of China
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To demonstrate mechanism of ESW in curing osteogetic disorders,we studied expression of some osteogenetic factors in human mesenchymal stem cells(hMSCs)when exposed to ESW.[Method]After success in marrow aspiration,isolation and obtainment optimal experimental energy,a dose of 5kV and 100 times of ESW was applied to hMSCs of passage 1.The expression of IGF-Ⅰ and TGF-?1 were examined by immunocytochemical staining.[Result]The cytochemical staining results showed that expression of IGF-Ⅰ and TGF-?1 appeared at different passage of hMSCs after ESW intervention.Appearance of IGF-Ⅰ was earlier than TGF-?1 which didnt express until passage 7.At the same interval,the expression of IGF-Ⅰ and TGF-?1 in control group difference is lower than ESW group,respectively(P
