Expression of interleukin-7 mRNA and identification of its splice variants
- VernacularTitle:白细胞介素7(IL-7)选择性剪接变异体的筛选和序列测定
- Author:
Deshun PAN
;
Peicheng WU
;
Fanhu KONG
;
Dixun WANG
- Publication Type:Journal Article
- Keywords:
IL-7;
Cloning;
Molecular;
Sequence analysis;
Carcinoma;
Splice variant
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of interleukin-7 in human liver and stomach,identify the splice variants of IL-7.Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used for investigating the expression of IL-7.According to IL-7 gene cDNA,the primers were designed and synthesized,then the splice variants of IL-7 in human cancer tissues was identify,cloned into vector and sequenced.Results:The results showed that(1)the expression of IL-7 mRNA was nearly equal between human liver and stomach,(2)two new bands of IL-7 was obtained from liver cancer and gastric carcinoma,one lacks exon 4,another exon 5.Conclusion:IL-7 gene is expressed highly in both human liver and stomach.In addition,human liver cancer and gastric carcinoma can produce splicing variants of IL-7 by alternative splicing.
