The research of p33~(ING1),wt-p53 growth suppressing and collapsing effect toward stomach cancer cell strain
- VernacularTitle:p33~(ING1)和wt-p53转染对胃癌细胞株生长抑制及凋亡的作用研究
- Author:
Furong WU
;
Houzhong DING
;
Kun FENG
;
Hai LI
;
Sijie ZHEN
;
Canrong NI
;
Guanzhen YU
- Publication Type:Journal Article
- Keywords:
p33~(IGN1);
wt-p53;
Stomach cancer cell strain;
Growth suppressing;
Collapse
- From:
Cancer Research and Clinic
1999;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the growth suppressing, apoptosing effect of new type tumor-supressor gene-p33ING1 in stomach cancer cell strain, and to explore new strategies and methods in tumor therapy. Methods The PCDNA3/p33ING1 nuclear expressing microsome was constructed, p33ING1 and wt-p53 were implanted to human stomach cancer cell both and to evaluate the effect of p33ING1 and p53 toward stomach cancer cell and synergism between them. Results The PCDNA3/p33ING1 nuclear expressing microsome was successfully constructed. The human stomach cancer cell strain SSCG-7901 under implantation of p33ING1 and wt-p53 showed a significant decrease in cell growth, the coupling time was delayed, DNA synthetic phase was shortened and G0/G1 phase prolonged. The cell collapse increased. Conclusions Despite of the tumor-inhibiting effect and biochemical activation of p33ING1, it also plays a role with p53 gene in controling growth of stomach cancer cell, inducing cell collapse and hampering cell proliferation cycle. P33ING1 and p53 are synergistic to each other.
