Dynamic detection of plasma cytomegalovirus DNA for predicting CMV pneumonia in renal transplant recipients
- VernacularTitle:动态检测血浆CMV DNA载量在预测肾移植术后并发巨细胞病毒肺炎中的意义
- Author:
Wentong ZENG
;
Qing YE
;
Guanghua LUO
;
Xuan DONG
;
Xiaozhou HE
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
Cytomegaloviruses;
Pneumonia
- From:
Chinese Journal of Urology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between cytomegalovirus pneumonia (CMV-IP) and viral load in renal transplant recipients and to find out the threshold value of viral load for predicting CMV-IP. Methods The blood samples of 56 renal transplant recipients were taken weekly for the first 2 months and every 2 weeks for 2-6 months after transplantation.Real-time PCR were used to quantify the plasma CMV DNA.The mean viral loads of CMV-IP group and non-CMV-IP group in each time were compared using Wilcoxon test.Different cut-off values were tested to find the suitable values to predict the CMV-IP. Results Of the 56 recipients,8 (14.3%) developed CMV-IP.The viral loads were near zero in the first 4 weeks in both groups;from week 5 the viral load of CMV-IP group increased gradually and reached the climax at week 8 and then declined,but the load of non-CMV-IP group fluctuated at a low level.During weeks 5-11,the viral loads of CMV-IP group were higher than those of non-VMV-IP group.At 5,7 and 9 weeks,the differences of the viral loads between the 2 groups were significant (P
