The effect of spironolactone on the expression of TGF-?_1 , PDGF-BB and ?-SMA in hepatic fibrosis tissues in rats
- VernacularTitle:螺内酯对实验大鼠肝组织TGF?1、PDGF-BB及?-SMA表达的影响
- Author:
Ai JIA
;
Xinming CHANG
;
Yingtao ZHANG
;
Yingchao LI
;
Ying CHANG
- Publication Type:Journal Article
- Keywords:
spironolactone;
hepatic fibrosis;
transforming gro wth factor- ?1;
platelet-derived growth factor-BB;
? -smooth muscle actin;
hepatic stellate cell (HSC)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preventive effect of spironolactone on hepatic fibrosis. Methods Ninety SD rats were randomly divided into three groups. Control group consisted of 8 rats that , fed by normal food, were injected with peanut oil subcutaneously. Model group consisted of 42 rats whose liver fibrosis was induced by compound factors. Spiro nolactone-prevention group consisted of 40 rats that were given 100 mg?kg -1 spironolactone per day, by the same methods of making models as those of the model group. At the end of weeks 2, 4, 6 and 8, 8 rats were randomly taken out of model group and spironol actone group and then were sacrificed. The expressions of TGF- ? 1 , PDGF- BB and ? -SMA in hepatic tissues were detected with immunohistochemical met hods. Results The expressions of TGF- ? 1 , PDGF-BB a nd ? -SMA in spironolactone group decreased greatly than those in model gro up ( P
