Observation of influence of FTY720 on peripheral blood lymphocytes and T cell subsets of mice in vivo
- VernacularTitle:FTY720对小鼠外周血淋巴细胞及T细胞亚群的影响
- Author:
Lei ZHANG
;
Liqun ZUO
;
Shiqian SHEN
- Publication Type:Journal Article
- Keywords:
lymphocytes;
FTY720;
T cell subsets
- From:
Chinese Journal of Urology
2000;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of FTY720 on the lymphocytes and their recovery after withdrawal of FTY720,and on the changes in T cell subsets of inbred mice so as to direct the administration of FTY720. Methods Male inbred C57/bl mice were given FTY720 at the dose of 3 mg/kg per day by oral gavage once a day for 14 days. The PBLs were counted and T cell subsets were analyzed by flow cytometry at the selected time points. Results When FTY720 was administrated, the PBLs showed a sharp decrease and reached the minimum 4 hours later.Then the PBLs remained stable during the whole pe- riod of administration.The PBLs didn’t restore immediately when the reagent was withdrawn,but increased slowly 2 weeks later and completely recovered 6 weeks later.The changes of T cells were similar to PBLs.CD - 8 subsets showed relatively higher sensitivity to FTY720. Conclusions FTY720 is a safe reagent which has rapid and specific effects on PBLs,and the changes in PBLs are reversible.The effect of FTY720 on T cell subsets is similar to that on PBLs.CD - 8 T cells are relatively more sensitive to FTY720.
