The inhibitory effect of c-myc antisense oligodeoxynucleotide on the growth of renal carcinoma cell and effect on cell cycle
- VernacularTitle:c-myc反义寡核苷酸对肾癌细胞的生长抑制作用及对细胞周期的影响
- Author:
Ziming WANG
;
Tie CHONG
;
Peng ZHANG
- Publication Type:Journal Article
- Keywords:
renal cell carcinoma;
c-myc;
antisense oligodeoxynucleotide
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effects of modification c-myc antisense oligodeoxynucleotide (ASODN) on the growth of renal carcinoma cell line (GRC-1) and cell cycle. Methods We transfected c-myc antisense oligodeoxynucleeride in renal carcinoma cell line to investigate the effect of c-myc ASODN on it. Results ① By MTT assay,we found that the c-myc ASODN significantly inhibited the growth of carcinoma cell, compared with sense and mismatch ODN. The growth-inhibition rate of 12 ?mol?L -1 c-myc ASODN group of renal carcinoma cell was 47% at 48 hour, and the inhibitory effect increased with the increase of ASODN dosage and changed with ASODN time. ② The measurements by flow cytometry proved that ASODN mainly blocked the G 1 to S of the cycling of renal carcinoma cell. Conclusion c-myc ASODN can inhibit the growth of renal carcinoma cells with sequence specificity and cycling specificity.
