The dose-effect relationship between active DNA and SLE-like syndrome and its characteristic
- VernacularTitle:活性DNA诱导小鼠SLE样综合征的量效关系及其特点
- Author:
Jin WU
;
Sidong XIONG
;
Housheng WU
- Publication Type:Journal Article
- Keywords:
Active DNA;
Systemic lupus erythematosus;
Anti-dsDNA antibody;
Anti-histone antibody
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the relationship between the dose of active DNA and the induction of SLE-like syndrome.Methods: DNA from extracted ConA-activated spleen lymphocytes and immunized syngenic mice with different quantities of active DNA, the anti-dsDNA antibodies and anti-histone antibodies as well as the antibody subclass were detected by ELISA.The patterns of antinuclear antibodies and immune complexes in glomeruli were observed by immunofluorescent-stain. Results: 10 pig active DNA could induce all the animal to produce anti-dsDNA and anti-histone antibodies,and the induced autoann'bodies were mainly IgGl type.Only 25%animals produced autoantibodies immunized by 5 fjig active DNA. Conclusion:The minimum dose of active DNA to induce SLE-like syndrome was 10 ^tg,and it predominantly evoked the humoral response.
