Construction of the Reporter Vector Controlled by Two Tandem Copies of Estrogen Response Elements in Yeast
- VernacularTitle:雌激素效应元件调控的酵母报告载体的构建
- Author:
Shihua HE
;
Zenghui LIANG
;
Wei ZHAN
- Publication Type:Journal Article
- Keywords:
Environmental estrogens;
Estrogen response elements;
Yeast;
Reporter gene
- From:
Journal of Environment and Health
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the recombinant yeast estrogen system(RYES),a reporter vector controlled by estro-gen response elements(ERE)in yeast was constructed.Methods pLacZi-1RER was obtained first by inserting an ERE which was synthesized artificially into the yeast reporter vector pLacZi which had already been lined by SalI and the terminal dephos-phorized by CIAP.Then,pLacZi-zERE.was obtained by inserting another ERE into the XhoI site nearby the ERE in pLacZi-1ERE.Results Sequence analysis indicated that the yeast reporter vector controlled by two tandem copies of ERE was con-structed successfully.Conclusion The yeast reporter vector controlled by ERE,pLacZi-2ERE,was constructed successfully,which was the very important groundwork for further establishment of the recombinant yeast estrogen system(RYES).
