Effect of G3PD modifier protein on the renal functions in aged rats with gentamycin-induced acute renal failure
- VernacularTitle:G3PD修饰蛋白对药物诱导的老年急性肾功能衰竭大鼠肾功能的影响
- Author:
Lan WU
;
Qing REN
;
Tingfu LI
- Publication Type:Journal Article
- Keywords:
Glyceraldehyde-3-phosphate dehydrogenases;
Amino acid motifs;
Nitric oxide;
Kidney failure, acute
- From:
Chinese Journal of Geriatrics
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether G3PD modifier protein (MP) could repair tubular epidermal cell(TEC) and ameliorate renal functions in aged rats with gentamycin induced acute tubular necrosis(ATN). Methods Aged and young rats were randomly divided into 3 groups, namely aged and young normal groups, aged and young model groups and the aged and young MP groups. The animal models of acute renal failure (ARF) were induced by gentamycin (140 mg?kg -1 ?d -1 , ip?7 d). Nitric oxide (NO) concentration was determined by Cd activated cadmium reduction method, malonaldehyde(MDA) with thiobarbituric acid test, superoxide dismutase(SOD) with hydroxylamine test, serum creatinine (Scr) with picric acid method, and the renal histology was observed by light and electron microscopy. Results NO was significantly higher in the aged MP group than that in the aged model group 〔serum: (94.29?7.68)?mol/L vs (70.14?5.53)?mol/L, P
