The immune reaction in mucosal and systemic immune system was induced after intranasal immunization with bivalent Shigella vaccines
- VernacularTitle:双价痢疾菌苗滴鼻免疫小鼠诱导粘膜与系统免疫反应
- Author:
Cuili SHU
;
Jieying GAO
;
Hong PENG
- Publication Type:Journal Article
- Keywords:
Shigella vaccine Intranasal immunization Lymphocytes phenotype Antigen specific antibody FACS
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect on different mucosal sites and system immune sites after intranasal immunization with bivalent Shigella vaccines Methods:BALB/c mice were divided into three groups at random , 10 mice per group Mice were intranasally immunized respectively with FSM 2117or FS 5416 (4?10 7CFU) three doses with an interval of two weeks The NALT, NP, spleen, PP, MLN, lymphocytes were isolated on the seventh day after the last immunization to assay the change of the cell phenotype with FACS The nasal ?lung?intestine?genital tract lavage fluid and serum were taken to assay the specific IgA or IgG against F2a or Sonni LPS with ELISA Results:The specific IgA and IgG in different mucosal sites and serum increased significantly after intranasal immunization with two Shigella vaccines compared with the control (P
