Production and Identification of Rat-Rat Monoclonal Anti-idiotypic Antibody against Anti-HBs
- VernacularTitle:大鼠抗——HB_s的单克隆抗独特型抗体杂交瘤细胞系的建立
- Author:
Shangxian GAO
- Publication Type:Journal Article
- Keywords:
Anti-idiotype antibody;
Monoclonal antibody;
HB vaccine;
Rat
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Two hybridomas (TD_1, TD_2)secreting monoclonal anti-idiotypic antibodies (anti-ld) againstanti-HBs were established by fusing the myeloma cells, IR983F, with the spleen cells of LOU/Crat which had been immunized with mouse monoclonal anti-HBs to the a determinates and syn-genic rat polyclonal anti-HBs. TD_2(Ab_2)showed the ability to inhibit the binding of horse anti-HBs with HBsAg and was also neutralized by anti-HBs from various origins. Ab_2 can induce an-ti-anti-ld (Ab_3)when injected it into LOU/C rats introperitoneally. Ab_3 can react specifically withHBsAg. These data indicate that TD_2 monoclonal anti-ld against anti-HBs are the anti-ld bearingthe internal image of HBsAg epitope that could mimic HBsAg and possess the immunogenicity ofHBsAg.
