Determination of IL-2 Content in IL-2 Targeting 5-FURP Liposomes by Ultraviolet Spectrophotometry
- VernacularTitle:紫外分光光度法测定IL-2靶向5-FURP脂质体中IL-2的含量
- Author:
Meichun WU
;
Yi ZHAO
;
Li ZENG
;
Chi WANG
- Publication Type:Journal Article
- Keywords:
IL-2;
Coomassie brillant blue G-250;
5′-palmitoyl-5-fluorouridine prodrug;
Content determination
- From:
China Pharmacy
2007;0(34):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of IL-2 in IL-2 targeting liposomes of 5-FURP. METHODS: The sample solution was stained by Coomassie brillant blue G-250 solution. Using blank liposome as blank,the absorbability of the IL-2 was determined by ultraviolet spectrophotometry at a wavelength of 595 nm;meanwhile its content was determined. RESULTS: The linear range of IL-2 was 1～100 ?g?ml-1(r=0.999 7) and the average recovery rate was 99.26% (RSD=0.59%,n=5). CONCLUSION: The method was proved to be simple,rapid,sensitive and reproducible,and it is applicable for the determination of IL-2 in liposome of 5-FURP.
