Effect of Tiopronin on Cytochrome P_(450)2E1 Expression in Liver of Rats with Alcoholic Fatty Liver
- VernacularTitle:硫普罗宁对酒精性脂肪肝大鼠肝组织细胞色素P_(450)2E1表达的影响
- Author:
Chengping JIANG
;
Bihua WU
- Publication Type:Journal Article
- Keywords:
Tiopronin;
Alcoholic fatty liver;
Rat;
Cytochrome P450 2E1
- From:
China Pharmacy
2007;0(28):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of tiopronin on the expression of cytochrome P4502E1(CYP2E1)in liver of rats with alcoholic fatty liver.METHODS:30 Wistar rats were randomly divided into 3 groups:group A(blank control group),group B(model group)and group C(tiopronin group).Group B and group C were given 50% alcohol intragastrically to establish alcoholic fatty liver model,while group C were intervened with tiopronin(0.15 g?kg-1?d-1 administered at 1 hour after alcohol administration)for 5 consecutive weeks.Then all the rats were sacrificed with AST and ALT levels in serum determined.The CYP2E1 expression was detected by RT-PCR;and the pathologic changes of the liver tissues were observed.RESULTS:In group B compared with group A,serum levels of AST,ALT and CYP2E1 expression were significantly higher(P
