Effect of bFGF on glucose regulated protein 78 expression and apoptosis in human ovarian cancer CAOV3 cells
- VernacularTitle:碱性成纤维细胞生长因子对人卵巢癌CAOV3细胞凋亡及葡萄糖调节蛋白78表达的影响
- Author:
Liping YE
;
Youfeng WEN
;
Ying ZHANG
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor 2;
Glucose regulated protein 78;
Protein kinase B;
Ovarian neoplasms;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the effect of bFGF on the apoptosis and the expression of glucose regulated protein 78(GRP78) in ovarian cancer CAOV3 cells.METHODS:Starvation induced cell apoptosis was conducted.After treatment with bFGF,the cell cycle and apoptosis were determined by FACS analysis and Annexin V/PI staining,respectively.The expression of protein kinase B(PKB) and GRP78 were detected by Western blotting and RT-PCR.RESULTS:Compared to starvation group,the cells treated with bFGF were still viable and increased activation of PKB and high expression of GRP78 were observed,which were prevented by PKB inhibitor wortmannin effectively(P
