Determination of Quercetin and Kaempferol in Platycladus orientalis Carbonisatus by RP - HPLC
- VernacularTitle:RP-HPLC法同时测定侧柏叶炭中槲皮素和山柰素的含量
- Author:
Huaien WU
;
Hanshen ZHEN
;
Zhiying WEI
;
Chenglin CHEN
- Publication Type:Journal Article
- Keywords:
RP - HPLC;
Platycladus orientalis carbonisatus;
Quercetin;
Kaempferol;
Content determination
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determinate the contents of quercetin and kaempferol in Platycladus orientalis carbonisatus by RP - HPLC.METHODS:The determination was performed on Lichrospher C_(18)(250 mm?4.6 mm,5?m) with mobile phase consisted of methanol-0.2%phosphoric acid solution(55:45) at a flow rate of 1.0 mL?min~(-1).The column temperature was set at 25℃and the detection wavelength was set at 368 nm.RESULTS:The linear ranges of quercetin and kaempferol were 0.24～1.68?g(r =0.999 9) and 0.052～0.624?g(r = 0.999 8),respectively.The average recovery rates of quercetin and kaempferol were 100.91%(RSD = 0.80%,n = 6)and 99.59%(RSD = 1.07%,n = 6),respectively.CONCLUSION:The method is simple,accurate,reproducible,and applicable for the quality control of Platycladus orientalis carbonisatus.
