Determination of Ursolic Acid in Radix et Rhizoma Clematidis by Capillary Zone Electrophoresis with High Frequency Conductance Method
- VernacularTitle:毛细管电泳高频电导法测定威灵仙中熊果酸的含量
- Author:
Jizhu LIU
;
Feng ZHANG
;
Fenyun SONG
- Publication Type:Journal Article
- Keywords:
Capillary electrophoresis;
High frequency electric conductance;
Radix et Rhizoma Clematidis;
Ursolic acid
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a capillary zone electrophoresis with high frequency conductance method for the determination of ursolic acid in Radix et Rhizoma Clematidis.METHODS:The separation of sample was performed on an uncoated fused silica capillary(60 cm?75 ?m ID,effective length:56 cm) with 1.2 mmol?L-1 triethylamine-HCl(pH 10.60) and 0.24 mmol?L-1 ?-cyclodextrin as running buffer solution.The separation voltage was 12.5 kV and the gravity injection of sample was conducted for 10 s at a height of 20 cm.RESULTS:The calibration curve of ursolic acid was linear over the concentration range of 5.55～111.0 mg?mL-1(r=0.999 3) and the average recovery for ursolic acid was 96.0%(RSD=1.39%,n=6). CONCLUSION:The method was proved to be simple,accurate,rapid and reproducible,and suitable for the quantitative determination of ursolic acid in Radix et Rhizoma Clematidis.
