Determination of Baicalin in Tanreqing Injection by HPLC
- VernacularTitle:HPLC法测定痰热清注射液中黄芩苷的含量
- Author:
Qichun HUANG
;
Wensheng TU
- Publication Type:Journal Article
- Keywords:
Tanreqing injection;
Baicalin;
HPLC;
Determination
- From:
China Pharmacy
2007;0(30):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of Baicalin in Tanreqing injection.METHODS: The determination was performed by HPLC using an CLC-ODS C18 column(150 mm?6.0 mm,5 ?m),with mobile phase consisted of methanol-water(47∶53) at a flow rate of 0.5 mL?min-1 and a detection wavelength of 280 nm.RESULTS: The calibration curve for Baicalin was linear in the concentration range of 2.53～12.65 ?g?mL-1(r=0.999 8).The average recovery was 99.61%(RSD=0.35%,n=9).CONCLUSION: The method is simple,rapid and applicable for the quality control of Tanreqing injection.
