Effect of LY294002 on Cell Cycle and Apoptosis of Multidrug Resistant HL60/VCR Cell Line
- VernacularTitle:LY294002对多药耐药急性白血病细胞株HL60/VCR的凋亡及细胞周期的影响研究
- Author:
Biyi HUANG
- Publication Type:Journal Article
- Keywords:
LY 294002;
Multidrug resistant;
HL60/VCR;
Apoptosis;
Cell cycle
- From:
China Pharmacy
2005;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of the specific inhibitor-LY294002 of the signaling pathway PI3K/AKT on cell cycle and apoptosis of HL60/VCR cells and its mechanism.METHODS:Vincristine(VCR) resistant HL60/VCR were treated with LY294002(final concentration at 1.0,2.5,5.0,or 10 ?mol?L-1) or without LY294002(control).IC50 was detected by MTT;flow cytometry was used to evaluate apoptosis and cell cycle distribution,and changes of Bcl-2,CyclinD1 gene mRNA levels were detected by reverse transcription polymerase chain reaction.RESULTS:As compared with control,LY294002 significantly reduced the IC50 value in HL60/VCR to vincristine in a concentration dependent manner(P
