Detection of Chemical Constituents in Antirheumatic Traditional Chinese Medicines
- VernacularTitle:抗风湿(炎)类中成药中添加化学成分的检验方法研究
- Author:
Pingping FU
;
Huijun LIU
;
Weiyi CHEN
;
Yuehan ZHANG
;
Qiuxiang DONG
;
Chunming DUAN
- Publication Type:Journal Article
- Keywords:
Antirheumatic traditional Chinese medicine;
Chemical constituents;
HPLC
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the detection of Prednisone acetate, Hydrocortisone, Dexamethasone acetate, Piroxicam, Diclofenac sodium, Indometacin and Ibuprofen, the 7 chemical constituents in antirheumatic traditional Chinese medicine. METHODS: The chromatographic separation was performed on a Hypersil BDS C18 column (200 mm?4.6 mm,5 ?m) using methanol-1 g?L-1KH2PO4(58∶42) (adjusted to pH 4.0?0.05 with H3PO4) as the mobile phase at a flow rate of 1.0 mL?min-1. The detective wavelength was set at 220 nm. RESULTS: Seven chemical constituents were all well-separated with good linearity within respective range of sample size. CONCLUSION: The method is rapid and convenient, and can be used for the quantitative and qualitative analyses of 7 chemical constituents in antirheumatic traditional Chinese medicine and to detect whether there is addition of chemical constituents in antirheumatic traditional Chinese medicines.
