Expression and distribution of osteopontin in the development of pulmonary hypertension induced by hypoxia-hypercapnia
- VernacularTitle:低氧和高二氧化碳作用下肺动脉高压形成过程中骨桥蛋白表达与分布的实验研究
- Author:
Quan LIN
;
Liangxing WANG
;
Xiajie LUO
;
Xiaoying HUANG
;
Yanfan CHEN
;
Shaoxian CHEN
;
Xiaofang FAN
- Publication Type:Journal Article
- Keywords:
Osteopontin;
Pulmonary hypertension;
Anoxia;
Hypercapnia
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression and distribution of osteopontin (OPN) in lungs and pulmonary arteries in pulmonary hypertensive rats induced by hypoxia-hypercapnia, and to explore the role of OPN in pathogenesis of pulmonary hypertension. METHODS: Forty-eight male Sprague-Dawley rats (Weight 180 g-220 g) were randomly divided into four groups: normal control group (NC), hypoxic hypercapnia 1-week,2-week and 4-week group (1HH, 2HH and 4HH). The expressions of OPN mRNA and protein in lungs and pulmonary arteries were detected by RT-PCR and immunohistochemistry. ELISA was used to detect the concentration of OPN in lung homogenates. The content of OPN in pulmonary arteries was detected by Western blotting. RESULTS: ① The mean pulmonary artery pressure (mPAP) and weight ratio of right ventricle to left ventricle and septum [RV/(LV+S)] in all hypoxic hypercapniac groups were higher than those in normal control group (P0.05). ② The expression of OPN mRNA was significantly increased in pulmonary arteries and lung tissues in hypoxic hypercapnic groups compared with normal control group (P
