Study of cotransfection of B7-1 gene and CD1_D gene in pancreatic carcinoma cell and its anti-tumor responses in mice
- VernacularTitle:CD1_D基因与B7-1基因共转染胰腺癌细胞及其抗癌作用的实验研究
- Author:
Kunhua WANG
;
Kunmei GONG
;
Yongxue ZHANG
;
Ming ZHONG
;
Yiming OUYANG
;
Ping LIN
;
Yingguang HUANG
;
Jian ZHANG
;
Yu ZHU
;
Weijun LIU
;
Xirong ZHAO
- Publication Type:Journal Article
- Keywords:
Genes,CD1D;
Genes,B7-1;
Transfection;
Pancreatic Carcinoma Cell
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cotransfection mB7-1 and mCD1D gene into pancreatic cancer cells of rats and to observe its anti-tmor responses.Methods Recombinant retroviral vectors expressing mB7-1and mCD1D gene were packaged into GP2-293 cell lines and transfected.The expressions of mB7-1 and mCD1D were detected with PCR and Western blot.The positive cells of mB7-1 and mCD1D were used to induce the anti-tumor immunity in vitro.Results Anti-tumor immunity was induced after B7-1 and CD1D positive cells were coinoculated in syngeneic mice.Furthermore,the growth of tumor was inhibited.Conclusions Cotransfection of B7-1 and CD1D could induce anti-tumor effect.This study provide a foundation for the application of B7-1 and CD1D gene therapy in tumor.
