Study on Extraction Technique for Xiangjiang Granula Essential Oil
- VernacularTitle:香姜颗粒挥发油的萃取工艺研究
- Author:
Haini LI
;
Siwang WANG
;
Jianbo WANG
;
Ying CHANG
;
Jianbo TU
;
Zhihua QI
- Publication Type:Journal Article
- Keywords:
Xiangjiang Granula;
Essential oil;
Ligustilide;
Supercritical-fluid extraction;
Orthogonal test
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the optimum supercritical- fluid extraction technique for Xiangjiang Granula essential oil. METHODS: The orthogonal test was adopted to optimize the extraction process using the content of Ligustilide and the yield rate of essential oil as indicators, and 95% ethanol as co- solvents. The content of Ligustilide was determined by HPLC, using Phenomenex Luna C18( 250nm? 4. 6nm, 5? m) as column and methanol- 0. 5% glacial acetic acid( 30∶ 70) as mobile phase, with the detection wavelength set at 280 nm. RESULTS: The optimal extraction conditions were: temperature at 50℃ , pressure at 45MPa, extraction time for 3h, and 95% ethanol as co- solvents. The Ligustilide had a good linearty relationship between 5. 1～ 25. 5? g? mL- 1( r=0. 999 8) . CONCLUSIONS: This technique is easy, convenient and workable, and can provide theoretical support for production.
