Pharmacodynamic Optimization of Purification Technique for Acanthopanax Senticosus Harms
- VernacularTitle:结合药效的刺五加纯化工艺优选
- Author:
Xiaobin TAN
;
Xiaobin JIA
;
Mingqin SHEN
;
Yunru PENG
- Publication Type:Journal Article
- Keywords:
Acanthopanax senticosus Harms;
Syringin;
Purification technique;
D-101 macroporous resin;
Cerebral ischemia
- From:
China Pharmacy
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize the purification technique for the extract of Acanthopanax senticosus Harms. METHODS: The techniques of D- 101 macroporous resin adsorption, polyamide adsorption and n- butanol extraction were separately applied to purify the aqueous extract of Acanthopanax senticosus Harms. Syringin was detected by HPLC and total flavone was detected by UV. The anti- cerebral ischemia effects of three purified samples were compared using rat MCAO model and mouse decapitation gape model. RESULTS: The three purified samples all showed anti- cerebral ischemia effects, with the sample purified via D- 101 macroporous resin adsorption having stronger effects than the other two. The content of Syringin was correlated with anti- cerebral ischemia effects. CONCLUSIONS: D- 101 macroporous resin adsorption is a be-tter technique for purifying the aqueous extract of Acanthopanax senticosus Harms.
