GM-CSF induces human vascular endothelial cells to form vessel-like structure and the role of VEGF
- VernacularTitle:GM-CSF对诱导人血管内皮细胞血管形成的影响及VEGF的作用
- Author:
Wei ZHAO
;
Xianghong YANG
;
Yanlin WANG
;
Yuezhong WANG
;
Yajia ZHANG
- Publication Type:Journal Article
- Keywords:
Granulocyte-macrophage colony stimulating factor;
Arteriosclerosis;
Endothelial growth factors;
Blood vessels
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the influence of GM-CSF on human vascular endothelial cells induced to form new blood vessels and the role of VEGF.METHODS: HUVECs were cultured by Matrigel to set up a stable angiogenesis system with the stimulating factors.The rhGM-CSF concentration-dependent and time-dependent effects and the role of VEGF165 were detected.CD34 was measured by immunochemical staining and numbers of vessel formation was calculated under microscopic observation.RESULTS: After treatment with rhGM-CSF at various concentrations and at different time points,the numbers of vessel formation increased in a dose-dependent and time-dependent manner.In the presence of VEGF165,the numbers of vessel formation increased evidently.CONCLUSION: HUVECs were induced to develop tubular structure in vitro cultured with Matrigel.GM-CSF promotes human vascular endothelial cells to form vessel-like structure in vitro in a dose-dependent and time-dependent manner.VEGF also in vitro promotes human vascular endothelial cells to form new vessel-like structure.
