Determination of 5-Fluorouracil in Magnetic Micropheres and Tissues in Mice by RP-HPLC
- VernacularTitle:RP-HPLC法测定5-Fu磁性微球及小鼠各组织中5-Fu的含量
- Author:
Shaojun SHI
;
Zhongfang LI
;
Huating CHEN
- Publication Type:Journal Article
- Keywords:
5-Fluorouracil;
Magnetic micropheres;
RP-HPLC;
Target therapy;
Tissue distribution
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a RP-HPLC method for the determination of 5-fluorouracil(5-Fu)in magnetic micropheres (MMS), and to evaluate the target ability of 5-Fu magnetic microspheres in mice. METHODS: 5-Fu-MMS was digested with 0.5% pepsin, and then free 5-Fu was extracted from tissue with ethyl acetate, and detected by a validated RP-HPLC method. RESULTS: The calibration curve was linear over the range of 0.1～25mg?L-1 and the limit of quantization was 0.1mg?L-1. The tissue distribution of 5-Fu-MMS in the liver was significantly increased as compared to control(P
