Effects of grasp seed procyanidins on homocysteine-induced proliferation and migration in vascular smooth muscle cells
- VernacularTitle:原花青素对同型半胱氨酸诱导的血管平滑肌细胞增殖、迁移的影响
- Author:
Weizhen XUE
;
Jiliang WANG
;
Zhaoxu MAO
;
Zhimin XU
;
Xiaoping CHEN
;
Zhenti DANG
;
Jie REN
;
Dengfeng MA
;
Yingjie TAI
;
Yigang HE
- Publication Type:Journal Article
- Keywords:
Grasp seed procyanidins;
Homocysteine;
Smooth muscle cells;
NF-kappa B;
Reactive oxygen species
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of grasp seed procyanidins(GSP) on homocysteine-induced proliferation and migration in vascular smooth muscle cells(VSMC) and related molecular mechanisms.METHODS: Cell count and -TdR assay were used for detecting cell proliferation and DNA synthesis,ELISA assay was used for detecting inflammatory response,DCFH-DA assay for examining the levels of reactive oxygen species(ROS),Western blotting for detecting protein expression.RESULTS: Homocysteine(0.1-1 mmol/L) increased VSMC proliferation and migration,and the levels of ROS were in a dose-dependent manner.The results of Western blotting showed that homocysteine significantly increased the expression of MCP-1,IL-6 and TNF-?.However,Compared with control group,in GSP(5-20 g/L) group,the increased VSMC proliferation,migration and the production of ROS and the expression of MCP-1,IL-6 and TNF-? mediated by homocysteine were markedly suppressed.EMSA showed that in GSP treatment group,the NF-?B activation was also almost completely inhibited.CONCLUSION: GSP inhibits homocysteine-induced VSMC proliferation,migration and inflammatory response through interfering with ROS dependent on NF-?B signal pathway.
