Inhibitory effect of triptolide on production of IL-1? from PBMC is associated with IL-1? gene polymorphism
- VernacularTitle:雷公藤甲素抑制PBMC分泌IL-1?蛋白量与IL-1?基因多态性有关(英文)
- Author:
Dongyun SHENG
;
Shenghao TU
;
Hongbo CHEN
;
Yonghong HU
- Publication Type:Journal Article
- Keywords:
Interleukin-1;
Genes;
Polymorphism(Genetics);
Tripterygium;
Arthritis,rheumatoid
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore whether the inhibitory effect of triptolide on IL-1? production by PBMC is associated with IL-1? gene polymorphisms.METHODS:IL-1? gene polymorphism was analyzed in 31 healthy volunteers.From genomic DNA,the C-T polymorphism at IL-1?-511 was typed by PCR-RFLP.Meanwhile the IL-1? was also measured in the supernatants of the cultured and stimulated peripheral blood mononuclear cells(PBMC)by ELISA.RESULTS:After LPS stimulation in PBMC cultures of healthy subjects,the secretion levels of IL-1? in 9 volunteers who carried IL-1?-511 T/T genotype were higher than in volunteers who are not T/T genotype(P0.05).CONCLUSION:The gene polymorphism at IL-1?-511 was related to the production of IL-1?,and the inhibitory effect of triptolide on the production of IL-1? was different in C/C,C/T,T/T genotype of IL-1?-511,which may be one of the reasons for the phenomenon that people respond differently to triptolide.
