Effects of H_2O_2 on growth and proliferation in CN2Z cells in vitro
- VernacularTitle:H_2O_2对人鼻咽癌细胞系CNE-2Z生长和增殖的影响
- Author:
Shoujun ZHONG
;
Yunhong YAO
;
Hui XIONG
;
Jianhua YU
- Publication Type:Journal Article
- Keywords:
H_2O_2;
Nasopharyngeal Carcinoma;
Cell culture;
Egr-1 gene;
Tumor related gene;
Tumor therapy
- From:
Journal of Chinese Physician
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of H_2O_2 on growth and proliferation in CNE-2Z cells.Methods To identify the effects of H_2O_2 on the growth and proliferation in CNE-2Z cells,Cell growth curves,MTT assay and plate colony formation rate were used;Meanwhile,Cell cycle distribution,proliferation index and apoptosis analysis were performed by flow cytometry(FCM) in human NPC cells CNE-2Z in vitro,before and after the action of H_2O_2;the protein expressions of Egr-1,p53,p16,Cyclin D1 were detected by Western-Blotting analysis.Results There were no EGR-1 and P16 proteins expressions in CNE-2Z cells by Western-Blotting analysis before the action of H2O2;the growth and proliferation of CNE-2Z cells were inhibited significantly by H_2O_2 compared with the control cells,and the apoptosis cells were increased.The increased expressions of EGR-1,P53,and Cyclin D1 protein were also shown in CNE-2Z cells by means of Western-Blotting analysis after the action of H_2O_2;but no P16 protein expression;The expression of EGR-1 protein was significant correlation with P53 protein expression,but no significant correlation with Cyclin D1 protein expression.Conclusion There was no EGR-1 protein expression in CNE-2Z cells,and EGR-1 protein can be induced expression rapidly and transiently by H_2O_2,which probably have an important!role in the growth inhibition and apoptosis.The promotor of Egr-1 gene also can be induced activation by radiation(ROS,reactive oxygen species),there is an important application in therapy of NPC and other tumors.
