Effects of peroxisome proliferators-activatied receptor gamma on the biological characteristics of hepatic stellate cells
- VernacularTitle:过氧化物酶体增殖物活化的受体?对肝星状细胞作用机制的研究
- Author:
Zhaohui ZHONG
;
Yantong GUO
;
Mai ZHOU
;
Gangjun JIAO
;
Xisheng LENG
;
Yuhua WEI
;
Xin YU
- Publication Type:Journal Article
- Keywords:
Heptocytes;
Peroxisome proliferators-actived receptors;
Rosiglitazone;
Gene expression
- From:
Chinese Journal of General Surgery
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of peroxisome proliferators-activated receptor gamma (PPA?r) on the biological characteristics of hepatic stellate cells (HSCs); Methods The activated HSCs were divided into four groups: control group, rosiglitazone group, GW9662 + rosiglitazone group and GW9662 group. The cell proliferation was determined with MTT colorimetric assay, The cell apoptosis was studied with flow cytometry. The expression of PPA?r, Type I collagen were detected by RT-PCR, Western blot and immunocytochemistry. Results MTT of HSCs in rosiglitazone group was (0. 49?0. 06) significantly lower than in control group ( 1. 00?0. 045 ), GW9662 group (0. 89?0. 043 ) , GW9662 + rosiglitazone group (0.78?0.049)(t = 15.59,14.68,8.07, P
