Effect of H_2O_2 on voltage-gated potassium channels in rat pulmonary artery smooth muscle cells
- VernacularTitle:H_2O_2对大鼠肺动脉平滑肌细胞Kv通道的作用
- Author:
Hongyan LUO
;
Ming TANG
;
Yimei DU
;
Changjin LIU
;
Bi TANG
;
Xinwu HU
;
Mouxian HU
;
Jiaoya XI
;
Yuanlong SONG
;
Hescheler JURGEN
- Publication Type:Journal Article
- Keywords:
Hydrogen peroxide;
Smooth muscle cells;
Potassium channels,voltage-gated;
Patch-clamp techniques
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the effect of hydrogen peroxide(H_2O_2) on voltage-gated potassium channel currents(IKv) in pulmonary vascular smooth muscle cells(PASMCs).METHODS: Using whole cell patch-clamp technique,IKv was recorded in freshly isolated rat PASMCs with acute enzymatic digestion method.The effect of hydrogen peroxide on IKv in PASMCs was investigated in normoxia.RESULTS: IKv in PASMCs was increased significantly by H_2O_2 and the increase depended on the concentration in normoxia.Current-voltage relationship curve shifted to the left.CONCLUSION: Hydrogen peroxide is an important K~+ channel opener.
