Detection of p53 and K-ras gene mutation in portal venous blood of gastric carcinoma patients
- VernacularTitle:胃癌患者门静脉血中p53和K-ras基因突变的检测及其临床意义
- Author:
Jixian CHEN
;
Jianwu HUANG
;
Jie ZHANG
;
Changming DOU
;
Hongzhi LI
;
Yongkang CHEN
;
Renhu ZHANG
;
Weili WU
;
Qian CHEN
;
Ming YU
;
Daozhe LIN
;
Feng LIN
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Gene mutation;
Neoplasm metastasis
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relations between p53 and K-ras gene mutation in portal venous blood of gastric carcinoma patients and cancer metastasis. Methods p53 and K-ras gene mutation was detected with PCR-SSCP technology in 62 cases of gastric carcinoma. Results p53 and K-ras mutation rate were 39% and 34% in portal venous blood, but only 8% and 4. 8% in peripheral blood; The rate of gene mutation in p53 and K-ras were 24% and 22% in patient without liver metastasis, 92% and 77% in patient with liver metastasis; The rate of gene mutation in p53 and K-ras in portal venous were 39% and 34% before surgical exploration, but 56% and 63% after exploration. The rate of positive detection of the mutation was significantly (P
