Protective effects of [Gly~(14)]-humanin on cultured neural stem cells treated with ?-amyloid protein in vitro
- VernacularTitle:[Gly~(14)]-humanin多肽对?淀粉样蛋白引起的神经干细胞毒性的拮抗作用研究
- Author:
Xianmin XU
;
Tingjie WANG
;
Xianjiu CHEN
;
Yuehong ZHANG
;
Niuliang CHENG
;
Dayong CAI
;
Jun XIE
;
Bo NIU
- Publication Type:Journal Article
- Keywords:
Stem cells;
Amyloid beta-protein;
Humanin peptide
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of [Gly~(14)]-humanin,one of the strongest derivate of humanin,on proliferation and differentiation of neural stem cells(NSCs) and the protective action of cell death or apoptosis induced by ?-amyloid protein 1-42.METHODS: NSCs were treated with different concentrations of [Gly~(14)]-humanin and ?-amyloid protein 1-42.RESULTS: 10 nmol/L [Gly~(14)]-humanin made NSCs resistant to the apoptotic action induced by A?P1-42 and prevented NSCs from death induced by 25 ?mol/L ?-amyloid protein 1-42.The differentiated neural stem cells yield more neuronal cells than that in control groups when 10 nmol/L [Gly~(14)]-humanin was added to the culture media.The number of cells increased and the cultures grown with a manner of floating cell clones likes that cultured in the presence of mitogen when 100 nmol/L [Gly~(14)]-humanin was added to the differentiation culture media.CONCLUSION: The [Gly~(14)]-humanin significantly promoted the proliferation and neuronal differentiation of neural stem/progenitor cells and also inhibited the toxic action of ?-amyloid protein 1-42 on cultured neural stem/progenitor cells.
