10-23 deoxyribozymes inhibit the expression of bacterial ?-lactamase gene
- VernacularTitle:10-23脱氧核酶抑制细菌?-内酰胺酶基因表达的实验研究
- Author:
Yongen XIE
;
Baodong LING
;
Xian YU
;
Changqing LI
;
Gang LIU
;
Xiang ZHANG
- Publication Type:Journal Article
- Keywords:
DNA, catalytic;
Lactamase;
Gene expression;
Bacteria
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inhibitory effects of 10-23 deoxyribozyme (10-23 DRz) on Escherichia coli ?-lactamase gene expression. METHODS: According to the gene sequence of Escherichia coli ?-lactamase gene blashv-5, 10-23 DRz and antisense oligonucleotides (As-ODN) were designed and synthesized. 10-23 DRz, As-ODN or control oligonucleotides were respectively introduced into Escherichia coli by the method of electroporation. Following electroporation, bacterial viability in liquid medium contained ceftazidime was detected, bacterial ?-lactamase expression was analysed by using IEF-PAGE and the ?-lactamase band was measured with gel documentation-analyzing system. RESULTS: A_ 600 in 10-23 DRz transfected Escherichia coli was lower compared with that in As-ODN transfected Escherichia coli (P
