Preparation of glycine liposomes and it's effect on cardiomyocyte injury induced by hypoxia/reoxygenation
- VernacularTitle:甘氨酸脂质体的制备及其对心肌细胞缺氧/复氧损伤的影响
- Author:
Mengfei CHEN
;
Daxiang LU
;
Renbin QI
;
Huadong WANG
;
Yanping WANG
;
Xue ZHAO
;
Chujie LI
- Publication Type:Journal Article
- Keywords:
Glycine;
Liposomes;
Cardiomyocytes;
Myocardial reperfusion injury
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To prepare glycine liposome microparticle and observe the effect of glycine liposomes on cardiomyocyte injury induced by hypoxia/reoxygenation. METHODS: (1) Reverse-phase evaporation method was used to produce glycine liposomes, the effects of different organic solvents: aether, chloroform and two mixtures of aether/chloroform on entrapment efficiency were evaluated, transmission electron microscope was used to detect the particle diameter of glycine liposomes. (2) A cardiomyocyte injury model was established by using hypoxia/reoxygenation, the activities of lactate dehydrogenase (LDH), creatine kinase (CK) and creatine kinase isoenzyme (CK-MB) of each group were detected. RESULTS: The entrapment efficiency of glycine liposomes prepared with the mixtures of aether/chloroform is highest compared with other organic solvents (64.8%, P
