Effects of decoy strategy targeted to NF-?B on interieukin-6 in rat's liver after traumatic inflammation
- VernacularTitle:核因子-?B“圈套”策略对创伤性炎症大鼠肝脏炎症介质IL-6的作用研究
- Author:
Wenjun YANG
;
Zhengping YU
;
Qiyu ZHANG
;
Huaping LIANG
;
Xiang XU
;
Qitong SONG
;
Guanbao ZHU
;
Feizhao JIANG
;
Hongqi SHI
- Publication Type:Journal Article
- Keywords:
Animal experimentation;
Wounds and injuries;
Liver;
NF-kappa B;
Interleukin-6
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of decoy strategy targeted to NF-?B on IL-6 in rat's liver after traumatic inflammatin. Methods Ninty six Wistar rats were randomly divided into 4 groups: control group,traumatic inflammation group, decoy ODN group, and mutant decoy ODN group. Rats were killed on 3 , 6, 12, 24, 48 , and 72 h respectively, for the determination of plasma ALT. Hepatocytes were isolated and nuclear protein was extracted, DNA binding activity of NF-?B was measured by EMSA. Decoy ODN's competition inhibition effect was assayed by EMSA. IL-6 gene expression in liver tissue was assessed by RT-PCR and IL-6 protein level was determined by ELISA. Results After traumatic inflammation, DNA binding activity of NF-?B in the liver increased. IL-6 mRNA and protein level also significantly increased and was in positive correlation with the activity of NF-?B. Decoy ODN effectively inhibited the activity of NF-?B ex vivo. After using decoy ODN, IL-6 mRNA and protein levels of liver tissue significantly decreased, plasma ALT levels were also significantly decreased. Conclusions Decoy strategy targeted to NF-?B could effectively inhibit rat's liver IL-6 release by inhibiting specifically the activity of NF-?B.
