Content Determination of Ribavirin and Diphenhydramine Hydrochloride in Flu Nasal Drops by HPLC
- VernacularTitle:高效液相色谱法测定感冒滴鼻剂中利巴韦林和盐酸苯海拉明的含量
- Author:
Lijuan ZHI
;
Yanhui LIU
;
Xiangwu KONG
;
Yaozen LIU
;
Xinghua WANG
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Flu nasal drops;
Ribavirin;
Diphenhydramine hydrochloride;
Content determination
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for content determination of ribavirin and diphenhydramine hy-drochloride in flu nasal drops.METHODS:The chromato bar was ZORBAX SB-CN,the mobile phase was acetonitrile-wa-ter-phosphoric acid(38∶62∶0.1),with the flow rate of1.0ml/min and the detection wave at224nm,the sample size was20?l.RESULTS:A good linear relationship were obtained between the peak areas and the concentrations of ribavirin and diph_ enhydramine hydrochloride respectively in the range from0.1～1.0mg/ml(r=0.9990)and0.02～0.2mg/ml(r=0.9930),the average recoveries were100.3%(RSD=0.62%,n=9)and100.8%(RSD=0.54%,n=9),respectively.CONCLUSI_ ON:This method is convenient,rapid,accurate,and brings about good recovery,it can be used for quality control of flu nasal drops.
