Expression of urotensinⅡ receptor GPR14 in aorta of apoE knockout mice
- VernacularTitle:apoE基因敲除小鼠主动脉尾加压素Ⅱ受体GPR14表达的变化
- Author:
Zhijian WANG
;
Wenhui DING
;
Libin SHI
;
Lei MENG
;
Ziwen REN
;
Dingfang PU
;
Yonggang ZHANG
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
Urotensins;
Receptors, G-protein-coupled;
Arteriosclerosis;
Genes, apoE
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
] AIM: To investigate the expression of the urotensin Ⅱ (UⅡ) receptor GPR14 in the aorta of apoE knockout mouse. METHODS: The expression of GPR14 in the aorta of apoE knockout C57BL/6J mice at various ages (18 weeks, 28 weeks, and 38 weeks old, respectively) was determined with competitive RT-PCR. A binding assay of [ 125 I]-UⅡ on the aortic tissue was also performed in 28 weeks group. RESULTS: We found significant upregulation of GPR14 mRNA at all three ages. Compared with wild type group at the same age, the GPR14 mRNA level in apoE knockout mice increased 54.2% in 18 week group (P
