Levels of IL-18, IL-16, IL-8, eotaxin and the chymase activity in the sputum of asthmatics
- VernacularTitle:哮喘患者痰中IL-18、IL-16、IL-8、嗜酸性粒细胞趋化因子水平和类糜蛋白酶活性的测定
- Author:
Hua XIE
;
Shaoheng HE
;
Jifu WEI
;
Li SUN
;
Ping CHEN
- Publication Type:Journal Article
- Keywords:
Chymase;
Interleukin-18;
Interleukin-16;
Interleukin-8;
Chemotactic factors, eosinophil;
Asthma;
Mast cells
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the levels of IL-18, IL-16, IL-8, eotaxin and the chymase activity in the sputum of asthmatics. METHODS: IL-18, IL-16, IL-8 and eotaxin levels were detected with sandwich ELISA procedures and chymase activity was determined spectrophotometrically (410 nm) by the rate of hydrolysis of N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide (SAAPP). RESULTS: The specific chymase activities in the severe and moderate asthmatics were higher than that in controls. Native protease inhibitors ?_1-antitrypsin (?_1-AT) and soybean trypsin inhibitor (SBTI) inhibited 71.9% and 72.1% enzymatic chymase activity, respectively. The levels of IL-18, IL-16, IL-8 and eotaxin were significantly elevated in the sputum of patients with acute asthma. There were correlations between the levels of IL-8 and IL-16 (r=0.55, P
