Effects of antioxidant and NF-?B on the induction of IL-8 in human colon cancer cell line HT-29 cells in vitro
- VernacularTitle:抗氧化剂和NF-?B对大肠癌细胞IL-8表达的作用及机制
- Author:
Wenpeng ZHOU
;
Jinmin WU
;
Xing ZHANG
;
Hongming PAN
;
Yong FANG
;
Houjun CAO
;
Hong WANG
;
Junping ZHANG
;
Zaiyun ZHANG
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Interleukin-8;
NF-kappa B;
Pyrrolidine dithiocarbamate
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of nuclear factor ?B (NF-?B) in the induction of IL-8 gene by TNF-? in colon cancer cells and the effect of antioxidant on the induction of IL-8. METHODS: ELISA was used to detect the concentrations of IL-8. IL-8 mRNA was analyzed by using RT-PCR. NF-?B in the cell nuclei was detected with electrophoretic mobility shift assay. RESULTS: (1) IL-8 production and IL-8 mRNA expression induced by TNF-? was blocked by pyrrolidine dithiocarbamate (PDTC). (2) TNF-? triggered the activation and translocation of NF-?B and PDTC inhibited the activation of NF-?B induced by TNF-?. CONCLUSION: The induction of IL-8 gene and protein by TNF-? is dependent on the activation of NF-?B. Antioxidants may inhibit the induction of IL-8 gene and protein through inhibiting NF-?B activation.
