Preparation and Characterization of Hybridoma Strains Secreting Natural IgM Against Keratin
- VernacularTitle:天然属性的抗角蛋白单克隆IgM抗体的制备
- Author:
Meng FU
;
Wei LI
;
Gang WANG
;
Yanguo ZHANG
;
Chengxin LI
;
Pingshen FAN
;
Yufeng LIU
- Publication Type:Journal Article
- Keywords:
Antibodies, monoclonal;
Immunoglobulin M;
Hybridomas;
Keratin
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare natural anti-keratin IgM monoclonal antibody. Methods Spleen cells of BALB/c mice raised in specific pathogen free conditions were directly fused with Sp2/0 myeloma cells. The hybridoma supernatants were tested by ELISA using pre-extracted keratin. The natural IgM obtained was further identified by immunochemistry and immunoblot methods. Results The cell fusion rate was about 60% without pre-immunization. About 14% supernatants reacted with the keratin antigen. Three hybridoma strains secreting natural IgM monoclonal antibody against keratin were obtained. The immunochemistry results showed that the natural anti-keratin IgM was able to bind to epidermis, sebaceous gland, hair follicule, and muscle tissues. Conclusion B lymphocytes in normal BALB/c mice spleen can produce natural antibody against kerain.
