Protective mechanism of 7-nitro indazole in neonatal rats with hypoxic-ischemic brain damage
- VernacularTitle:7硝基吲唑在缺氧缺血性脑损伤中的作用机制
- Author:
Guilan CHU
;
Shuying BI
;
Yue XIN
- Publication Type:Journal Article
- Keywords:
Indoles;
Hypoxia ischemia, brain;
Apoptosis
- From:
Chinese Journal of Perinatal Medicine
1998;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective mechanism of 7-nitro indazole (7-NI) in neonatal rats with hypoxic-ischemic brain damage. Methods Seventy-two Wistar rats of 7-day-old were randomly divided into the sham-operation、HIBD and 7-NI treated group. The HIBD models were established in 7-NI treated and HIBD groups.7-NI (100 mg/kg) or peanut oil (10 ml/kg) was injected intraperitoneally 0.5 h before hypoxia in 7-NI treated group or HIBD group. NOS,NO,SOD and MDA were examined at different time (1 h、2 h、6 h、8 h). Terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling(TUNEL) method was used to detect neuronal apoptosis. Results NOS and NO level of HIBD group at different time were significantly higher than that of the sham-operation group [NOS:1 h (3.345?0.367)U/mg, 8 h (4.469?0.275) U/mg vs (1.555?0.223) U/mg; NO: 1 h (2.419?0.254) ?mol/g, 8 h (3.556?0.309) ?mol/g vs (0.749?0.135) ?mol/g, P
