Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction
- VernacularTitle:Kv1.2、Kv1.5、Kv2.1钾通道在缺氧性肺血管收缩中的作用
- Author:
Yimei DU
;
Ming TANG
;
Changjin LIU
;
Zhigang HONG
;
Qinmei KE
;
Jiufang DI
;
Hongyan LUO
;
Mouxian HU
;
Xinwu HU
;
Jiaoya XI
;
Bi TANG
;
Heschele JURGEN
- Publication Type:Journal Article
- Keywords:
Pulmonary artery;
Potassium channels;
Patch clamp techniques;
Antibodies
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the role of Kv1 2, Kv1 5, Kv2 1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1 2/Kv1 5/Kv2 1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells [from (-51 8?0 8) mV to (-47 2? 0 7) mV, P
