In vitro perfusion method during cardiac graft preservation and its application in adenovirus mediated gene transfection
- VernacularTitle:小鼠心脏移植保存期间经体外灌注行重组腺病毒基因转染的研究
- Author:
Sheng YAN
;
Jiren YU
;
Ronghua ZHANG
;
Zili YANG
;
Qing ZHANG
;
Lei GUO
;
Tingbo LIANG
;
Shuse ZHENG
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Mice;
Adenovirus;
Gene transfection
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a method of in vitro donor heart perfusion in murine cardiac transplantation during preservation and apply it in adenovirus mediated gene transfection for donor heart. METHODS: Donor heart was transfected with recombinant adenovirus and stored for 2 hours after harvest, then it was transplanted heterotopically in abdomen. The grafts were appraisal by palpitation. Marked gene products were determined by X-Gal staining, aod T cell infiltration was determined by immunohistochemistry. The activation markers of recipients' lymphocytes were examed by cytometry. RESULTS: The grafts survival rate is 100% after perfusion and cold storage. The LacZ staining became strong 1 week after transplantation. The grafts remained an intact structure and no apparent T cell infiltration. The activation status of recipients' lymphocytes were not enhanced by transfected cardiac graft. CONCLUSION: In vitro perfusion during graft cold preservation is feasible for adenovirus mediated gene transfection. [
