Study of cytotoxicity of anti-CD20 monoclonal antibody against myeloma cells in vitro
- VernacularTitle:体外研究抗CD20单克隆抗体对骨髓瘤细胞的细胞毒性作用
- Author:
Zhenhai ZHOU
;
Juan LI
;
Yunxian CHEN
;
Shaokai LUO
;
Xiaoyin LI
;
Wende HONG
- Publication Type:Journal Article
- Keywords:
Multiple myeloma;
Antigens, CD20;
Antibodies, CD20;
Interferon-gamma, recombinant;
Antibody-dependent cell-mediated cytotoxicity
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the cytotoxicity on myeloma cells mediated by anti-CD20 monoclonal antibody-mabthera, after heightening level of CD20 expression on myeloma cells membrane by ?-interferon. METHODS: 10 untreated(UT) and 10 relapsed or refractory(RR) MM patients'myeloma cells were cultured with human recombinant ?-interferon (hr?-IFN) at concentrations of (0-800)?10~3 U/L to heighten level of CD20 expression, then complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC) on myeloma cells mediated by mabthera were studied through MTT methods. RESULTS: When CD20 expression of UT MM and RR MM patients' myeloma cells increased after treated by hr?-IFN, 12 mg/L and 16 mg/L mabthera mediated ADCC and CDC (against) myeloma cells in group UT patients and group RR patients, respectively. CONCLUSION: After heightened level of CD20 expression on myeloma cells membrane by hr?-IFN, mabthera mediated ADCC and CDC against myeloma cells in vitro.
