Analysis of doxepin in urine and whole blood by HPLC after solid-phase extraction
- VernacularTitle:多虑平SPE-HPLC分析方法的建立及其应用
- Author:
Chen LIANG
;
Yurong ZHANG
;
Jieru JIAO
- Publication Type:Journal Article
- Keywords:
Forensic toxicological analysis;
Doxepin;
SPE;
HPLC;
Urine;
Whole blood
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide a specific,sensitive,reproducible HPLC method for the determination of doxepin in urine and whole blood with doxapram as the internal standard.Methods The sample preparation was simple and time-saving by using an Oasis pretreatment cartridge.Separations were achieved by a Lichrospher 100 RP-18e (250mm?4.0mm,5?m)column kept at 50 ℃,and the DAD detector was set at 230nm and 250nm.Results The detection limit was 2 ng/ml in urine and whole blood.The results showed excellent linearity.The relative standard deviation of between-day and within-day assay was Less than 6.75%,and absolute recoveries were higher than 85%.Conclusion The method for determination of doxepin in urine and whole blood is effective,simple and reliable,which may be applicable to the practical cases.
